Scientific Research in Japan

Genetic Molecular Markers

Molecular tools and genetic markers for the generation of transgenic sexing strains in Anopheline mosquitoes. 

Molecular tools and genetic markers for the generation of transgenic sexing strains in Anopheline mosquitoes.

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Malaria is a important worldwide effectively being burden, affecting higher than 200 million people yearly in over 90 nations, predominantly in Africa, Asia and the Americas. Since the 12 months 2000, a concerted effort to struggle malaria has lowered its incidence by higher than 40%, totally on account of the use of insecticide-treated bednets, indoor residual spraying and artemisinin-based combination drug therapies.

Nevertheless, the value of administration is anticipated to only about triple over the subsequent decade and the current downward improvement in sickness transmission is threatened by the rise of resistance to treatment and pesticides. Novel strategies which is likely to be sustainable and cost-effective are wished to help usher in an interval of malaria elimination.

The easiest strategies so far have focussed on administration of the mosquito vector. The sterile insect strategy (SIT) is a most likely extremely efficient approach that targets to suppress mosquito populations by means of the unproductive mating of wild female mosquitoes with sterile males which is likely to be launched en masse.

The strategy and its derivatives are presently not relevant for malaria administration because of this of it is robust to sterilise males with out compromising their potential to mate, and because of this of anopheline males cannot be merely separated from females, which if launched, may contribute to sickness transmission.

Advances in genome sequencing utilized sciences and the progress of transgenic strategies current the tools important to provide mosquito sexing strains, which promise to boost current malaria-control packages and pave the strategy for new ones. In this consider, the progress made in the progress of transgenic sexing strains for the administration of Anopheles gambiae, a severe vector of human malaria, is talked about.

Molecular tools and genetic markers for the generation of transgenic sexing strains in Anopheline mosquitoes.
Molecular tools and genetic markers for the generation of transgenic sexing strains in Anopheline mosquitoes.

Molecular variation and inhabitants development in endangered Limonium bicolor: genetic selection of microsatellite markers and amplified fragment measurement polymorphism analysis.

Knowledge and analysis of the genetic development of an endangered species is important for its conservation and evolutionary course of. Simple sequence repeats (SSRs) and amplified fragment measurement polymorphisms (AFLPs) had been used in evaluation of the genetic selection and inhabitants differentiation in Limonium bicolor (Plumbaginaceae), an endangered herb with extreme medicinal and horticulture price.

CD4(EDU-2) Antibody
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Description: Primary antibody against CD4(EDU-2), CF405M conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC040345-100 100uL
EUR 199.00
Description: Primary antibody against CD4(EDU-2), CF405S conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC040345-500 500uL
EUR 544.00
Description: Primary antibody against CD4(EDU-2), CF405S conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC400345-100 100uL
EUR 199.00
Description: Primary antibody against CD4(EDU-2), CF640R conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
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EUR 544.00
Description: Primary antibody against CD4(EDU-2), CF640R conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC430345-100 100uL
EUR 199.00
Description: Primary antibody against CD4(EDU-2), CF543 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC430345-500 500uL
EUR 544.00
Description: Primary antibody against CD4(EDU-2), CF543 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC470345-100 100uL
EUR 199.00
Description: Primary antibody against CD4(EDU-2), CF647 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC470345-500 500uL
EUR 544.00
Description: Primary antibody against CD4(EDU-2), CF647 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC550345-100 100uL
EUR 199.00
Description: Primary antibody against CD4(EDU-2), CF555 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC550345-500 500uL
EUR 544.00
Description: Primary antibody against CD4(EDU-2), CF555 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC610345-100 100uL
EUR 199.00
Description: Primary antibody against CD4(EDU-2), CF660R conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC610345-500 500uL
EUR 544.00
Description: Primary antibody against CD4(EDU-2), CF660R conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC680345-100 100uL
EUR 199.00
Description: Primary antibody against CD4(EDU-2), CF568 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC680345-500 500uL
EUR 544.00
Description: Primary antibody against CD4(EDU-2), CF568 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC700345-100 100uL
EUR 199.00
Description: Primary antibody against CD4(EDU-2), CF770 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC700345-500 500uL
EUR 544.00
Description: Primary antibody against CD4(EDU-2), CF770 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC800345-100 100uL
EUR 199.00
Description: Primary antibody against CD4(EDU-2), CF680 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC800345-500 500uL
EUR 544.00
Description: Primary antibody against CD4(EDU-2), CF680 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC810345-100 100uL
EUR 199.00
Description: Primary antibody against CD4(EDU-2), CF680R conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC810345-500 500uL
EUR 544.00
Description: Primary antibody against CD4(EDU-2), CF680R conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC880345-100 100uL
EUR 199.00
Description: Primary antibody against CD4(EDU-2), CF488A conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC880345-500 500uL
EUR 544.00
Description: Primary antibody against CD4(EDU-2), CF488A conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC940345-100 100uL
EUR 199.00
Description: Primary antibody against CD4(EDU-2), CF594 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNC940345-500 500uL
EUR 544.00
Description: Primary antibody against CD4(EDU-2), CF594 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNCA0345-250 250uL
EUR 383.00
Description: Primary antibody against CD4(EDU-2), APC conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNCAP0345-100 100uL
EUR 199.00
Description: Primary antibody against CD4(EDU-2), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNCAP0345-500 500uL
EUR 544.00
Description: Primary antibody against CD4(EDU-2), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNCB0345-100 100uL
EUR 199.00
Description: Primary antibody against CD4(EDU-2), Biotin conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNCB0345-500 500uL
EUR 544.00
Description: Primary antibody against CD4(EDU-2), Biotin conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNCH0345-100 100uL
EUR 199.00
Description: Primary antibody against CD4(EDU-2), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNCH0345-500 500uL
EUR 544.00
Description: Primary antibody against CD4(EDU-2), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNCP0345-250 250uL
EUR 383.00
Description: Primary antibody against CD4(EDU-2), PerCP conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNCR0345-250 250uL
EUR 383.00
Description: Primary antibody against CD4(EDU-2), RPE conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody
BNUB0345-100 100uL
EUR 209.00
Description: Primary antibody against CD4(EDU-2), Concentration: 0.2mg/mL

CD4(EDU-2) Antibody
BNUB0345-500 500uL
EUR 458.00
Description: Primary antibody against CD4(EDU-2), Concentration: 0.2mg/mL

CD4(EDU-2) Antibody
BNUM0345-50 50uL
EUR 395.00
Description: Primary antibody against CD4(EDU-2), 1mg/mL

CytoSelect EdU Competitive ELISA Kit
CBA-5101 96 assays
EUR 543.00

EdU (5-ethynyl-2'-deoxyuridine), 100 mg
10540 100 mg
EUR 112.00

EdU (5-ethynyl-2'-deoxyuridine), 1 g
40540 1 g
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EdU (5-ethynyl-2'-deoxyuridine), 500 mg
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EdU (5-ethynyl-2'-deoxyuridine), 250 mg
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30007 1KIT
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Description: Minimum order quantity: 1 unit of 1KIT

Endothelial Tube Formation Assay (In Vitro Angiogenesis Assay)
CBA-200 50 assays
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Description: For angiogenesis to occur, endothelial cells must escape their stable location and break through the basement membrane. Cells migrate toward an angiogenic stimulus that may be released from nearby tumor cells. These cells proliferate to form new blood vessels. Our Endothelial Tube Formation Assay (In Vitro Angiogenesis) provides an easy, robust system to assess angiogenesis in vitro. The ECM gel matrix very closely resembles an in vivo environment.

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AKR-360 96 assays
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K415-200
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Proteasome Assay Kit
55R-1341 100 assays
EUR 654.00
Description: Assay Kit for detection of Proteasome in the research laboratory

Calpain Assay Kit
55R-1342 100 assays
EUR 779.00
Description: Assay Kit for detection of Calpain in the research laboratory

Glutathione Assay Kit
55R-1343 100 assays
EUR 654.00
Description: Assay Kit for detection of Glutathione in the research laboratory

Glutathione Assay Kit
55R-1354 100 assays
EUR 895.00
Description: Assay Kit for detection of Glutathione activity in the research laboratory

HDAC Assay Kit
55R-1371 100 assays
EUR 673.00
Description: Assay Kit for detection of HDAC in the research laboratory

HDAC Assay Kit
55R-1372 100 assays
EUR 673.00
Description: Assay Kit for detection of HDAC in the research laboratory

HAT Assay Kit
55R-1373 100 assays
EUR 740.00
Description: Assay Kit for detection of HAT in the research laboratory

SOD Assay Kit
55R-1374 100 assays
EUR 602.00
Description: Assay Kit for detection of SOD in the research laboratory

HDAC3 Assay Kit
55R-1377 100 assays
EUR 693.00
Description: Assay Kit for detection of HDAC3 in the research laboratory

HDAC8 Assay Kit
55R-1379 100 assays
EUR 689.00
Description: Assay Kit for detection of HDAC8 in the research laboratory

ATP Assay Kit
55R-1380 100 assays
EUR 809.00
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ADP Assay Kit
55R-1381 100 assays
EUR 809.00
Description: Assay Kit for detection of ADP in the research laboratory

FAD Assay Kit
55R-1382 100 assays
EUR 732.00
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PEP Assay Kit
55R-1384 100 assays
EUR 876.00
Description: Assay Kit for detection of PEP in the research laboratory

Ammonia Assay Kit
55R-1388 100 assays
EUR 740.00
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cAMP Assay Kit
55R-1389 100 assays
EUR 706.00
Description: Assay Kit for detection of cAMP activity in the research laboratory

cGMP Assay Kit
55R-1390 100 assays
EUR 706.00
Description: Assay Kit for detection of cGMP activity in the research laboratory

Urea Assay Kit
55R-1391 100 assays
EUR 740.00
Description: Assay Kit for detection of Urea in the research laboratory

Calcium Assay Kit
55R-1392 250 assays
EUR 586.00
Description: Assay Kit for detection of Calcium activity in the research laboratory

Magnesium Assay Kit
55R-1393 100 assays
EUR 654.00
Description: Assay Kit for detection of Magnesium in the research laboratory

Zinc Assay Kit
55R-1394 100 assays
EUR 673.00
Description: Assay Kit for detection of Zinc in the research laboratory

Iron Assay Kit
55R-1395 100 assays
EUR 758.00
Description: Assay Kit for detection of Iron in the research laboratory

Phosphate Assay Kit
55R-1400 500 assays
EUR 415.00
Description: Assay Kit for detection of Phosphate in the research laboratory

JNK Assay Kit
55R-1407 40 assays
EUR 868.00
Description: Assay Kit for detection of JNK in the research laboratory

JNK Assay Kit
55R-1408 40 assays
EUR 979.00
Description: Assay Kit for detection of JNK in the research laboratory

Akt Assay Kit
55R-1409 40 assays
EUR 979.00
Description: Assay Kit for detection of Akt in the research laboratory

Ammonia Assay Kit
55R-1410 100 assays
EUR 740.00
Description: Assay Kit for detection of Ammonia in the research laboratory

Cobalt Assay Kit
55R-1435 100 assays
EUR 621.00
Description: Assay Kit for detection of Cobalt in the research laboratory

Nickel Assay Kit
55R-1436 100 assays
EUR 621.00
Description: Assay Kit for detection of Nickel in the research laboratory

Chloride Assay Kit
55R-1437 100 assays
EUR 484.00
Description: Assay Kit for detection of Chloride in the research laboratory

Aspartate Assay Kit
55R-1438 100 assays
EUR 673.00
Description: Assay Kit for detection of Aspartate in the research laboratory

Hydroxyproline Assay Kit
55R-1439 100 assays
EUR 844.00
Description: Assay Kit for detection of Hydroxyproline in the research laboratory

Phenylalanine Assay Kit
55R-1442 100 assays
EUR 654.00
Description: Assay Kit for detection of Phenylalanine in the research laboratory

Phosphatidylcholine Assay Kit
55R-1443 100 assays
EUR 673.00
Description: Assay Kit for detection of Phosphatidylcholine in the research laboratory

Glucose Assay Kit
55R-1449 100 assays
EUR 706.00
Description: Assay Kit for detection of Glucose in the research laboratory

Lactate Assay Kit
55R-1450 100 assays
EUR 844.00
Description: Assay Kit for detection of Lactate in the research laboratory

Pyruvate Assay Kit
55R-1452 100 assays
EUR 844.00
Description: Assay Kit for detection of Pyruvate in the research laboratory

Adipogenesis Assay Kit
55R-1454 100 assays
EUR 586.00
Description: Assay Kit for detection of Adipogenesis in the research laboratory

Glucose Assay Kit
55R-1458 100 assays
EUR 740.00
Description: Assay Kit for detection of Glucose in the research laboratory

Galactose Assay Kit
55R-1459 100 assays
EUR 740.00
Description: Assay Kit for detection of Galactose in the research laboratory

Maltose Assay Kit
55R-1460 100 assays
EUR 723.00
Description: Assay Kit for detection of Maltose in the research laboratory

Fructose Assay Kit
55R-1461 100 assays
EUR 689.00
Description: Assay Kit for detection of Fructose in the research laboratory

Ethanol Assay Kit
55R-1462 100 assays
EUR 774.00
Description: Assay Kit for detection of Ethanol in the research laboratory

Galactose Assay Kit
55R-1463 100 assays
EUR 706.00
Description: Assay Kit for detection of Galactose in the research laboratory

Lactose Assay Kit
55R-1466 100 assays
EUR 706.00
Description: Assay Kit for detection of Lactose in the research laboratory

Creatinine Assay Kit
55R-1467 100 assays
EUR 638.00
Description: Assay Kit for detection of Creatinine in the research laboratory

Sucrose Assay Kit
55R-1468 100 assays
EUR 689.00
Description: Assay Kit for detection of Sucrose in the research laboratory

Lactate Assay Kit
55R-1469 100 assays
EUR 844.00
Description: Assay Kit for detection of Lactate in the research laboratory

Maltose Assay Kit
55R-1470 100 assays
EUR 706.00
Description: Assay Kit for detection of Maltose in the research laboratory

Glutamate Assay Kit
55R-1471 100 assays
EUR 740.00
Description: Assay Kit for detection of Glutamate in the research laboratory

Fumarate Assay Kit
55R-1475 100 assays
EUR 706.00
Description: Assay Kit for detection of Fumarate in the research laboratory

Creatine Assay Kit
55R-1476 100 assays
EUR 654.00
Description: Assay Kit for detection of Creatine in the research laboratory

Sarcosine Assay Kit
55R-1477 100 assays
EUR 654.00
Description: Assay Kit for detection of Sarcosine in the research laboratory

Malate Assay Kit
55R-1478 100 assays
EUR 673.00
Description: Assay Kit for detection of Malate in the research laboratory

Glycogen Assay Kit
55R-1481 100 assays
EUR 844.00
Description: Assay Kit for detection of Glycogen in the research laboratory

Starch Assay Kit
55R-1482 100 assays
EUR 673.00
Description: Assay Kit for detection of Starch in the research laboratory

Alanine Assay Kit
55R-1483 100 assays
EUR 654.00
Description: Assay Kit for detection of Alanine in the research laboratory

Formate Assay Kit
55R-1484 100 assays
EUR 809.00
Description: Assay Kit for detection of Formate in the research laboratory

Citrate Assay Kit
55R-1485 100 assays
EUR 740.00
Description: Assay Kit for detection of Citrate in the research laboratory

Isocitrate Assay Kit
55R-1486 100 assays
EUR 663.00
Description: Assay Kit for detection of Isocitrate in the research laboratory

Oxaloacetate Assay Kit
55R-1488 100 assays
EUR 663.00
Description: Assay Kit for detection of Oxaloacetate in the research laboratory

Heme Assay Kit
55R-1492 100 assays
EUR 673.00
Description: Assay Kit for detection of Heme in the research laboratory

Glucose Assay Kit
55R-1494 100 assays
EUR 706.00
Description: Assay Kit for detection of Glucose in the research laboratory

Amylase Assay Kit
55R-1503 100 assays
EUR 551.00
Description: Assay Kit for detection of Amylase in the research laboratory

Inosine Assay Kit
55R-1504 100 assays
EUR 732.00
Description: Assay Kit for detection of Inosine in the research laboratory

Aconitase Assay Kit
55R-1505 100 assays
EUR 689.00
Description: Assay Kit for detection of Aconitase in the research laboratory

Lipase Assay Kit
55R-1506 100 assays
EUR 706.00
Description: Assay Kit for detection of Lipase in the research laboratory

Lipase Assay Kit
55R-1507 100 assays
EUR 740.00
Description: Assay Kit for detection of Lipase in the research laboratory

MDA Assay Kit
55R-1512 100 assays
EUR 809.00
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Myeloperoxidase Assay Kit
55R-1514 100 assays
EUR 809.00
Description: Assay Kit for detection of Myeloperoxidase in the research laboratory

Myeloperoxidase Assay Kit
55R-1515 100 assays
EUR 758.00
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Asparaginase Assay Kit
55R-1518 100 assays
EUR 758.00
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Trypsin Assay Kit
55R-1524 100 assays
EUR 621.00
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Catalase Assay Kit
55R-1525 100 assays
EUR 570.00
Description: Assay Kit for detection of Catalase in the research laboratory

DPP4 Assay Kit
55R-1526 100 assays
EUR 750.00
Description: Assay Kit for detection of DPP4 in the research laboratory

Protease Assay Kit
55R-1528 100 assays
EUR 519.00
Description: Assay Kit for detection of Protease in the research laboratory

MMP3 Assay Kit
55R-1530 100 assays
EUR 723.00
Description: Assay Kit for detection of MMP3 in the research laboratory

PAH Assay Kit
55R-1547 100 assays
EUR 740.00
Description: Assay Kit for detection of PAH in the research laboratory

Glycosaminoglycans Assay Kit
6022 1 kit
EUR 419.75
Description: Glycosaminoglycans Assay Kit

DNA Assay Kit
6023 1 kit
EUR 180.50
Description: DNA Assay Kit

Hemoglobin Assay Kit
6024 1 kit
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Description: Hemoglobin Assay Kit

Catalase Assay Kit
abx294001-96tests 96 tests
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  • Shipped within 5-12 working days.

Hexokinase Assay Kit
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Ceruloplasmin Assay Kit
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Catalase Assay Kit
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Peroxidase Assay Kit
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Glutaminase Assay Kit
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Hydroxyproline Assay Kit
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Acetylcholinesterase Assay Kit
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EUR 441.00
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Citrate Assay Kit
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Hexokinase Assay Kit
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Phosphofructokinase Assay Kit
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Trypsin Assay Kit
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Pepsin Assay Kit
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Chymotrypsin Assay Kit
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Lipase Assay Kit
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Triglyceride Assay Kit
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Pectinase Assay Kit
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Chitinase Assay Kit
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Cellulase Assay Kit
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Sorbitol Assay Kit
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Kalium Assay Kit
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Iron Assay Kit
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Magnesium Assay Kit
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Phosphorus Assay Kit
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Natrium Assay Kit
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Zinc Assay Kit
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Copper Assay Kit
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Pyruvate Assay Kit
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NADPase Assay Kit
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Starch Assay Kit
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Trehalose Assay Kit
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ADA Assay Kit
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Calcium Assay Kit
abx098414-Hitachi7020R140ml2R240ml2 Hitachi 7020; R1: 40ml×2 R2: 40ml×2
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Calcium Assay Kit
abx098414-Hitachi7060R190ml1R290ml1 Hitachi 7060; R1: 90ml×1 R2: 90ml×1
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Calcium Assay Kit
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Calcium Assay Kit
abx098414-UniversalR140ml2R240ml2 Universal; R1: 40ml×2 R2: 40ml×2
EUR 206.00
  • Shipped within 5-12 working days.

Cholinesterase Assay Kit
abx098416-Hitachi7170R120ml1R25ml1 Hitachi 7170; R1: 20ml×1 R2: 5ml×1
EUR 300.00
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Cholinesterase Assay Kit
abx098416-Hitachi7170R140ml3R230ml1 Hitachi 7170; R1: 40ml×3 R2: 30ml×1
EUR 316.00
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Cholinesterase Assay Kit
abx098416-Hitachi7170R160ml2R230ml1 Hitachi 7170; R1: 60ml×2 R2: 30ml×1
EUR 316.00
  • Shipped within 5-12 working days.

Cholinesterase Assay Kit
abx098416-Toshiba40R140ml4R240ml1 Toshiba 40; R1: 40ml×4 R2: 40ml×1
EUR 331.00
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Cholinesterase Assay Kit
abx098416-UniversalR160ml2R215ml2 Universal; R1: 60ml×2 R2: 15ml×2
EUR 316.00
  • Shipped within 5-12 working days.

An entire of 117 alleles had been detected with a imply 5.85 alleles per locus using SSR and 222 bands from AFLP had been amplified in six populations. It was found that L. bicolor was characterised by extreme ranges of genetic polymorphism (100 and 83.78%), low ranges of complete genetic selection (Ht = 0.2824 and 0.2424), and common ranges of genetic differentiation amongst populations (ΦST = 0.284 and 0.251). Analysis of molecular variance (AMOVA) revealed that the main variation component existed inside populations (71.56%; 74.93%) moderately than amongst populations (28.44%; 25.07%).

Four main clusters had been displayed in the UPGMA using TFPGA, which was in retaining with the consequence of principal coordinate analysis (PCA) using NTSYS. Mutations or uncommon gene transfer amongst populations can enhance the plant slowly, thus in situ conservation insurance coverage insurance policies should be carried out first for environment friendly and sustainable progress. At the similar time, ex situ measures, corresponding to those individuals with unusual alleles, to take care of the relationships between individuals and populations are moreover proposed.

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