Genetic Diversity Studies and Identification of Molecular and Biochemical Markers Associated with Fusarium Wilt Resistance in Cultivated Faba Bean (Vicia faba).

Faba bean (Vicia faba L.) is one of the most important legume crops in Egypt. However, production of faba bean is affected by several diseases including fungal diseases. Fusarium wilt incited by Fusarium oxysporum Schlecht. was shown to be the most common wilt disease of faba bean in Assiut Governorate.

Evaluation of 16 faba bean genotypes for the resistance to Fusarium wilt was carried out under greenhouse conditions. Three molecular marker systems (inter-simple sequence repeat [ISSR], sequence related amplified polymorphism [SRAP], and simple sequence repeat [SSR]) and a biochemical marker (protein profiles) were used to study the genetic diversity and detect molecular and biochemical markers associated with Fusarium wilt resistance in the tested genotypes.

The results showed that certain genotypes of faba bean were resistant to Fusarium wilt, while most of the genotypes were highly susceptible. The percentage of disease severity ranged from 32.83% in Assiut-215 to 64.17% in Misr-3. The genotypes Assiut-215, Roomy-3, Marut-2, and Giza-2 were the most resistant, and the genotypes Misr-3, Misr-1, Assiut-143, Giza-40, and Roomy-80 performed as highly susceptible.

The genotypes Assiut-215 and Roomy-3 were considered as promising sources of the resistance to Fusarium wilt. SRAP markers showed higher polymorphism (82.53%) compared with SSR (76.85%), ISSR markers (62.24%), and protein profile (31.82%). Specific molecular and biochemical markers associated with Fusarium wilt resistance were identified. The dendrogram based on combined data of molecular and biochemical markers grouped the 16 faba bean genotypes into three clusters.

Cluster I included resistant genotypes, cluster II comprised all moderate genotypes and cluster III contained highly susceptible genotypes.

 Genetic Diversity Studies and Identification of Molecular and Biochemical Markers Associated with Fusarium Wilt Resistance in Cultivated Faba Bean (Vicia faba).
Genetic Diversity Studies and Identification of Molecular and Biochemical Markers Associated with Fusarium Wilt Resistance in Cultivated Faba Bean (Vicia faba).

Diversity Analysis of Sweet Potato Genetic Resources Using Morphological and Qualitative Traits and Molecular Markers.

The European Union (EU) market for sweet potatoes has increased by 100% over the last five years, and sweet potato cultivation in southern European countries is a new opportunity for the EU to exploit and introduce new genotypes. In view of this demand, the origins of the principal Italian sweet potato clones, compared with a core collection of genotypes from Central and Southern America, were investigated for the first time. This was accomplished by combining a genetic analysis, exploiting 14 hypervariable microsatellite markers, with morphological and chemical measurements based on 16 parameters.

From the molecular analyses, Italian accessions were determined to be genetically very similar to the South American germplasm, but they were sub-clustered into two groups. This finding was subsequently confirmed by the morphological and chemical measurements.

CytoSelect MTT Cell Proliferation Assay

CBA-252 960 assays
EUR 409
Description: Cell Biolabs? CytoSelect MTT Cell Proliferation Assay provides a colorimetric format for measuring and monitoring cell proliferation.  The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates or 192 assays in 24-well plates.  Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then detected with the proliferation reagent, which is converted in live cells from the yellow tetrazole MTT to the purple formazan form by a cellular reductase (Figure 1).  An increase in cell proliferation is accompanied by an increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

BrdU Cell Proliferation Assay Kit

C0300-050 500 Assays
EUR 928

BrdU Cell Proliferation Assay Kit

C0300-100 1000 Assays
EUR 1312

BrdU Cell Proliferation Assay Kit

C0300-500 5000 Assays
EUR 4438

BrdU Cell Proliferation Assay Kit

K306-1000
EUR 974

BrdU Cell Proliferation Assay Kit

K306-200
EUR 392

Cell Proliferation Assay Kit (Fluorometric)

K307-1000
EUR 408

CytoSelect Cell Proliferation Assay Reagent (Fluorometric)

CBA-250 10 mL
EUR 409
Description: Cell Biolabs? CytoSelect Cell Proliferation Assay Reagent (Fluorometric) provides a fluorometric format for measuring and monitoring cell proliferation. Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then incubated with the proliferation reagent.  Upon entering metabolically active live cells, the non-fluorescent proliferation reagent is converted into a bright red fluorescent form. An increase in cell proliferation is accompanied by increased fluorescent signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. The kit contains sufficient reagents for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates.

CytoSelect Cell Proliferation Assay Reagent (Colorimetric)

CBA-253 10 mL
EUR 409
Description: Cell Biolabs? CytoSelect WST-1 Cell Proliferation Assay Reagent provides a colorimetric format for measuring and monitoring cell proliferation.  The 10 mL volume is sufficient for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates.  Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then detected with the proliferation reagent, which is converted in live cells from WST-1 to the formazan form in the presence of cellular NADH and an electron mediator. An increase in cell proliferation is accompanied by increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

CellCount-Blue Cell Proliferation Assay Kit

C0100-025 250 Assays
EUR 111

CellCount-Blue Cell Proliferation Assay Kit

C0100-050 500 Assays
EUR 134

CellCount-Blue Cell Proliferation Assay Kit

C0100-100 1000 Assays
EUR 188

CellCount-Blue Cell Proliferation Assay Kit

C0100-500 5000 Assays
EUR 533

Cell Proliferation Assay Kit, WST-1

C0200-025 250 Assays
EUR 219

Cell Proliferation Assay Kit, WST-1

C0200-050 500 Assays
EUR 313

Cell Proliferation Assay Kit, WST-1

C0200-100 1000 Assays
EUR 469

MTT Cell Proliferation Assay Kit (Colorimetric)

K299-1000
EUR 316

MTS Cell Proliferation Colorimetric Assay Kit

K300-10000
EUR 1643

MTS Cell Proliferation Colorimetric Assay Kit

K300-250
EUR 196

MTS Cell Proliferation Colorimetric Assay Kit

K300-2500
EUR 577

MTS Cell Proliferation Colorimetric Assay Kit

K300-500
EUR 289

MTS Cell Proliferation Colorimetric Assay Kit

K300-5000
EUR 985

Quick Cell Proliferation Colorimetric Assay Kit

K301-2500
EUR 599

Quick Cell Proliferation colorimetric Assay Kit

K301-500
EUR 294

EZClick? EdU Cell Proliferation/DNA Synthesis Kit (FACS/Microscopy), Red Fluorescence

K946-100
EUR 533

MTT Cell Proliferation and Cytotoxicity Assay Kit

AR1156 1 kit (for 500 assays)
EUR 96

CytoX-Red Cell Proliferation/Cytotoxicity Assay Kit

OP-0004 10x96 assays
EUR 395.1
Description: reagents widely cited

CytoX-Violet Cell Proliferation/Cytotoxicity Assay Kit

OP-0005 20x96 assays
EUR 395.1
Description: fast delivery possible

MTT Cell Proliferation and Cytotoxicity Assay Kit

abx090676-500tests 500 tests
EUR 237

MTT Cell Proliferation and Viability Assay Kit

6034 1 kit
EUR 266.05
Description: MTT Cell Proliferation and Viability Assay Kit

Cell Proliferation and Cytotoxicity MTT Assay Kit

C0210-100 100 Assays
EUR 165

Cell Proliferation and Cytotoxicity MTT Assay Kit

C0210-500 500 Assays
EUR 300

Cell Proliferation and Cytotoxicity MTT Assay Kit

C0210-501 5000 Assays
EUR 1238

WST-1 Cell Proliferation Colorimetric Assay Kit

K2021-480 480 assays
EUR 321

WST Cell Proliferation Colorimetric Assay Kit plus

K2022-2500 2500 assays
EUR 599

WST Cell Proliferation Colorimetric Assay Kit plus

K2022-500 500 assays
EUR 321

Quick Cell Proliferation colorimetric Assay Kit Plus

K302-2500
EUR 599

Quick Cell Proliferation colorimetric Assay Kit Plus

K302-500
EUR 289

Neutral Red Cell Proliferation and Cytotoxicity Assay Kit

AR1157 1 kit (for 500 assays)
EUR 101

WST-1 Cell Proliferation and Cytotoxicity Assay Kit

AR1158 1 kit (for 100 assays)
EUR 86

WST-1 Cell Proliferation and Cytotoxicity Assay Kit

AR1159 1 kit (for 500 assays)
EUR 131

CD4(EDU-2) Antibody

BNCA0345-250 250uL
EUR 383
Description: Primary antibody against CD4(EDU-2), APC conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNCAP0345-100 100uL
EUR 199
Description: Primary antibody against CD4(EDU-2), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNCAP0345-500 500uL
EUR 544
Description: Primary antibody against CD4(EDU-2), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC810345-100 100uL
EUR 199
Description: Primary antibody against CD4(EDU-2), CF680R conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC810345-500 500uL
EUR 544
Description: Primary antibody against CD4(EDU-2), CF680R conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC880345-100 100uL
EUR 199
Description: Primary antibody against CD4(EDU-2), CF488A conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC880345-500 500uL
EUR 544
Description: Primary antibody against CD4(EDU-2), CF488A conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNCB0345-100 100uL
EUR 199
Description: Primary antibody against CD4(EDU-2), Biotin conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNCB0345-500 500uL
EUR 544
Description: Primary antibody against CD4(EDU-2), Biotin conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC550345-100 100uL
EUR 199
Description: Primary antibody against CD4(EDU-2), CF555 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC550345-500 500uL
EUR 544
Description: Primary antibody against CD4(EDU-2), CF555 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC430345-100 100uL
EUR 199
Description: Primary antibody against CD4(EDU-2), CF543 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC430345-500 500uL
EUR 544
Description: Primary antibody against CD4(EDU-2), CF543 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC470345-100 100uL
EUR 199
Description: Primary antibody against CD4(EDU-2), CF647 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC470345-500 500uL
EUR 544
Description: Primary antibody against CD4(EDU-2), CF647 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC940345-100 100uL
EUR 199
Description: Primary antibody against CD4(EDU-2), CF594 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC940345-500 500uL
EUR 544
Description: Primary antibody against CD4(EDU-2), CF594 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNCP0345-250 250uL
EUR 383
Description: Primary antibody against CD4(EDU-2), PerCP conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC040345-100 100uL
EUR 199
Description: Primary antibody against CD4(EDU-2), CF405S conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC040345-500 500uL
EUR 544
Description: Primary antibody against CD4(EDU-2), CF405S conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC050345-100 100uL
EUR 199
Description: Primary antibody against CD4(EDU-2), CF405M conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC050345-500 500uL
EUR 544
Description: Primary antibody against CD4(EDU-2), CF405M conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNCR0345-250 250uL
EUR 383
Description: Primary antibody against CD4(EDU-2), RPE conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNUB0345-100 100uL
EUR 209
Description: Primary antibody against CD4(EDU-2), Concentration: 0.2mg/mL

CD4(EDU-2) Antibody

BNUB0345-500 500uL
EUR 458
Description: Primary antibody against CD4(EDU-2), Concentration: 0.2mg/mL

CD4(EDU-2) Antibody

BNC680345-100 100uL
EUR 199
Description: Primary antibody against CD4(EDU-2), CF568 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC680345-500 500uL
EUR 544
Description: Primary antibody against CD4(EDU-2), CF568 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC700345-100 100uL
EUR 199
Description: Primary antibody against CD4(EDU-2), CF770 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC700345-500 500uL
EUR 544
Description: Primary antibody against CD4(EDU-2), CF770 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC800345-100 100uL
EUR 199
Description: Primary antibody against CD4(EDU-2), CF680 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC800345-500 500uL
EUR 544
Description: Primary antibody against CD4(EDU-2), CF680 conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC400345-100 100uL
EUR 199
Description: Primary antibody against CD4(EDU-2), CF640R conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC400345-500 500uL
EUR 544
Description: Primary antibody against CD4(EDU-2), CF640R conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC610345-100 100uL
EUR 199
Description: Primary antibody against CD4(EDU-2), CF660R conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNC610345-500 500uL
EUR 544
Description: Primary antibody against CD4(EDU-2), CF660R conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNCH0345-100 100uL
EUR 199
Description: Primary antibody against CD4(EDU-2), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNCH0345-500 500uL
EUR 544
Description: Primary antibody against CD4(EDU-2), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

CD4(EDU-2) Antibody

BNUM0345-50 50uL
EUR 395
Description: Primary antibody against CD4(EDU-2), 1mg/mL

CytoSelect EdU Competitive ELISA Kit

CBA-5101 96 assays
EUR 543

EdU (5-ethynyl-2'-deoxyuridine), 100 mg

10540 100 mg
EUR 112

EdU (5-ethynyl-2'-deoxyuridine), 250 mg

20540 250 mg
EUR 167

EdU (5-ethynyl-2'-deoxyuridine), 1 g

40540 1 g
EUR 435

EdU (5-ethynyl-2'-deoxyuridine), 500 mg

30540 500 mg
EUR 275

Anti-Proliferation Marker antibody

STJ16101069 100 µg
EUR 354

β cell proliferation agonist WS3

HY-12462 10mM/1mL
EUR 163

Proliferation-associated protein 2G4 Protein

20-abx260573
  • EUR 2861.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 25 ug
  • 5 ug

CLC/Ipomoea Medium, Callus Proliferation

CP005-010 10X1L
EUR 99

CLC/Ipomoea Medium, Callus Proliferation

CP005-500 50L
EUR 126

ViaFluor CFSE Cell Proliferation Kit

30050 1KIT
EUR 172
Description: Minimum order quantity: 1 unit of 1KIT

Rat Neurosphere-Progenitors Proliferation Media

M36111 500 ml
EUR 826

XTT CELL VIABILITY ASSAY KIT (1000 ASSAY)

30007 1KIT
EUR 236
Description: Minimum order quantity: 1 unit of 1KIT

Endothelial Tube Formation Assay (In Vitro Angiogenesis Assay)

CBA-200 50 assays
EUR 560
Description: For angiogenesis to occur, endothelial cells must escape their stable location and break through the basement membrane. Cells migrate toward an angiogenic stimulus that may be released from nearby tumor cells. These cells proliferate to form new blood vessels. Our Endothelial Tube Formation Assay (In Vitro Angiogenesis) provides an easy, robust system to assess angiogenesis in vitro. The ECM gel matrix very closely resembles an in vivo environment.

Lactose Assay Kit

MET-5001 100 assays
EUR 432
Description: The Lactose Assay Kit measures total lactose in milk based food products or biological samples such as blood or urine. Lactose is cleaved into glucose and galactose. Glucose is then oxidized, yielding hydrogen peroxide and D-gluconic acid. The hydrogen peroxide is detected by a fluorometric probe.

Bilirubin Assay Kit

MET-5010 200 assays
EUR 479
Description: Bilirubin, a byproduct of heme breakdown, can exist conjugated to glucuronic acid (direct) and as unconjugated (indirect). The unconjugated form is found in the blood bound to albumin and is transported to the liver. Bilirubin becomes conjugated to glucuronic acid in the liver, making it more soluble and allowing for excretion into bile. High levels of bilirubin have been correlated with jaundice and Gilbert?s syndrome while low levels have been associated with cardiovascular disease and diabetes mellitus.

Pyruvate Assay Kit

MET-5029 100 assays
EUR 479
Description: Our Pyruvate Assay Kit measures pyruvate in biological samples. First, pyruvate is oxidized by pyruvate oxidase, producing hydrogen peroxide. The hydrogen peroxide is then detected at ex. 530-570 nm/em. 590-600 nm using a specific fluorometric probe. Pyruvate levels in unknown samples are determined based on the provided pyruvate standard curve.

Glycine Assay Kit

MET-5070 100 assays
EUR 450

Taurine Assay Kit

MET-5071 200 assays
EUR 508

Moreover, the analysis of the genetic structure of the population suggested that one of the two groups of Italian genotypes may have descended from one of the South American accessions, as predicted on the basis of the shared morphological characteristics and molecular fingerprints.

Overall, the combination of two different characterization methods, genetic markers and agronomic traits, was effective in differentiating or clustering the sweet potato genotypes, in agreement with their geographical origin or phenotypic descriptors. This information could be exploited by both breeders and farmers to detect and protect commercial varieties, and hence for traceability purposes.

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